Journal: Journal of neurochemistry

Article Title: Putrescine-modified nerve growth factor: bioactivity, plasma pharmacokinetics, blood-brain/nerve barrier permeability, and nervous system biodistribution.

doi: 10.1046/j.1471-4159.1998.71041651.x

Figure Lengend Snippet: FIG. 1. Electrophoretic analysis of NGF and PUT-NGF modified at different pH values. A: SOS-PAGE (15% T, 1% C) of Coomas- sie Blue-stained gel. Native NGF shows a molecular weight of the monomer at 13,000 and the dimer at 26,000. NGFwas modi- fied with PUT at pH values of 5.7, 6.0, 6.4, and 6.7 and revealed the shifted molecular weight of the monomeric species and the presence of two new species at ‘—18,000 and ‘--28,000. All pro- teins were electrophoresed at 10 jig/lane. B: Western blot analy- sis after SDS-PAGE comparing immunoreactivity of native NGF versus NGF modified with PUT at pH 5.7, 6.0, 6.4, and 6.7 (1 jig of protein/lane). C: IEF analysis following Coomassie Blue stain. Native NGF shows a p1 of 8.0, and PUT-NGF shows a p1 of 9.6 after modification with PUT at the different pH values. In addition to the standards, the p1 values of cytochrome C (CYT C) and albumin (ALB) are also shown.

Article Snippet: IEF standards were obtained from Bio-Rad; they contained nine proteins with isoelectric points ranging from 4.45 to 9.6 and were visualized with Coomassie Blue.

Techniques: Modification, Staining, Molecular Weight, Western Blot, SDS Page